Author(s): WANG, S., XU, C.Y., Institution: SCHOOL OF SPORT SCIENCE, Country: CHINA, Abstract-ID: 1542

INTRODUCTION:
Obesity causes metabolic disorders in the body leading to chronic inflammation, with macrophage activation as the main feature at the site of inflammation. Abnormal accumulation of intrahepatic fat and infiltration of inflammatory cells form the pathological basis of liver inflammation. Aerobic exercise(AE) and calorie restriction(CR)can reduce intrahepatic fat deposition and decrease the degree of hepatocellular inflammation. Therefore, AE combined with CR may achieve better weight loss, and this effect may be consistent with improving the type of macrophage polarization. In the study, we intends to explore the possible mechanism of AE and CR for anti-inflammation and reduce weight from the perspective of macrophage polarization.
METHODS:
40 male 18 weeks db/db mice were randomly divided into four groups of 10 mice each: control group (OC),AE group, CR group, and AE combined with CR group (ECR). AE group used 50-55% VO2max intensity treadmill exercise for 12 weeks（60 mins,5 times per week）; the CR was given 70% of the intake of the OC daily; and the ECR was a combination of the two modalities with an intervention. Serum TC, TG LDL-C,HDL-C and Glu levels were measured in mice. The expression of iNOS, Arg-1, IL-1β, and IL-10 proteins in mouse liver tissues was detected by Western Blot.
RESULTS:
Body weight was significantly lower in the CR, AE and ECR than OC after intervention (P<0.01). The liver tissue weight of mice in the CR, AE and OER was significantly lower (P<0.01) than OC and ECR was significantly lower (P<0.01) than AE . Compared to OC, CR and ECR had a significant decrease in serum TC and LDL-C levels (P<0.05, P<0.01), compared to AE group mice, ECR had a significant decrease in TC, LDL-C (p<0.05, p<0.01); compared with the OC, the iNOS content in the livers of CR, AE was significantly reduced (p<0.05), and the iNOS content in the ECR was very significantly reduced (p<0.01), and there was a trend towards a decrease in the iNOS content in the ECR compared with the CR and AE (p>0.05); and compared with the OC, Arg-1 in the livers of AE, ECR content was significantly higher (p<0.05), and there was a trend of higher iNOS content in the ECR compared to the CR and AE (p>0.05); and compared with the OC group, CR, AE IL-10 content in liver was significantly elevated (p<0.05), and IL-10 content in ECR group was very significantly elevated (p<0.01); IL-10 levels were significantly higher in the ECR group compared to the CR and AE groups (p<0.05);there was no significant change in IL-1β content of AE, CR, and ECR compared to the OC, but there was a tendency to decrease.
CONCLUSION:
AE,CR, and the combined intervention of the two can reduce the weight gain of obese mice, reduce the weight of liver tissues, and improve lipid metabolism, probably by regulating the macrophage polarization status of liver tissues in obese mice, reducing M1 and increasing M2, thus reducing the inflammatory state of liver tissues.