Author(s): PERISSIOU, M., CORBETT, J., SAYNOR, Z.L., SHEPHERD, A.I., Institution: UNIVERSITY OF PORTSMOUTH, Country: UNITED KINGDOM, Abstract-ID: 2185

INTRODUCTION:
Ingestion of exogenous ketones (β-hydroxybutyrate; β-HB) through commercial ketone monoester (Kme) provides a rapid increase in blood β-HB levels [1] and has been shown to improve cardiac [2] and vascular function [3] in healthy individuals. Importantly, an increase in blood β-HB levels has been shown to improve cardiac function in people with heart failure [4]. However, the dose response relationship between the volume of Kme ingested and the corresponding cardiovascular effects invoked has yet to be investigated. In order to establish this relationship for clinical population, we need to first profile the pharmacokinetics of Kme in healthy individuals.
METHODS:
Ten healthy participants (age 21±2 yrs; BMI 23.6 kg/m2) completed a double-blind placebo-controlled trial. Participants attended the lab in four separate occasions and ingested Kme drink ((R)-3-hydroxybutyl (R)-3-hydroxybutyrate) of 0.1 g/kg, 0.4 g/kg, 0.8 g/kg or a placebo drink in a randomised order. Subsequently, participants underwent measurements of circulating [ßHb], cardiac output index (Qi) and stroke volume index (SVi) via thoracic impedance cardiography and flow mediated dilatation (FMD) via ultrasound at 30 min, 1h, 2h, 3h, 6h, 12h and 24h after drink ingestion.
RESULTS:
Data on circulating [ßHb], Qi, SVi and FMD will be presented comparing all different conditions over 24h in order to establish the effects of Kme on circulating ßHb and cardiovascular health.
CONCLUSION:
Establishing the dose-response relationship between Kme intake and cardiovascular function at rest will provide important information regarding the optimal Kme dose for improving cardiovascular health.

1. Cox, P.J., et al., Nutritional Ketosis Alters Fuel Preference and Thereby Endurance Performance in Athletes. Cell Metab, 2016. 24(2): p. 256-68.
2. Oneglia, A.P., et al., Acute effects of β-hydroxybutyrate on left ventricular function in young, healthy adults. J Appl Physiol (1985), 2023. 135(6): p. 1440-1445.
3. Walsh, J.J., H. Neudorf, and J.P. Little, 14-Day Ketone Supplementation Lowers Glucose and Improves Vascular Function in Obesity: A Randomized Crossover Trial. J Clin Endocrinol Metab, 2021. 106(4): p. e1738-e1754.
4. Nielsen, R., et al., Cardiovascular Effects of Treatment With the Ketone Body 3-Hydroxybutyrate in Chronic Heart Failure Patients. Circulation, 2019. 139(18): p. 2129-2141.