Author(s): DOUZI, W., BON, D., BIEUZEN, F., DUGUÉ, B., Institution: UNIVERSITE DE POITIERS , Country: FRANCE, Abstract-ID: 1354

INTRODUCTION:
Physical exercise induces various metabolic changes, influencing energy expenditure and substrate utilization. Metabolomics provides a comprehensive understanding of the metabolic adaptations occurring in response to physical exercise and recovery
METHODS:
Urine metabolomics with 1H-NMR spectroscopy was used to investigate the impact of sub-maximal cycling bout (SMC) in healthy young athletes at 60 % of their peak aerobic power on urine metabolome before and after a 4-week of high intensity interval training (HIIT) with or without the use of whole-body cryostimulation (WBC) as a recovery technique after each training session (WBC group, N=11; control group (CTL), N=12).
RESULTS:
Principal component analysis (PCA) revealed a distinct separation between the urine NMR profiles of WBC and CTL groups induced by SMC. However, these changes appeared to be more pronounced in the CTL group. Targeted analyses revealed that, prior to HIIT, SMC significantly increased lactate and adenine levels in CTL group compared to WBC group. SMC resulted also in a substantial reduction in citrate and formate levels only in CTL group before HIIT. Post- HIIT, alanine level increased only in WBC group, while N-phenylacetylglutamine decreased in the WBC group compared to CTL group. However, in both groups, there was a notable increase in lactate, acetate and adenine levels accompanied by a significant decrease in formate level.
CONCLUSION:
The SMC-induced changes following HIIT seem to be influenced by the repeated exposure to cryostimulation. The metabolic adaptations associated with SMC and HIIT contribute to a more comprehensive understanding of their combined effects and offer a distinctive perspective for unraveling the effects of physical activity and recovery on metabolic pathways.