INTRODUCTION:
Haemoglobin, a key oxygen-transporting protein in red blood cells, is an established biomarker of aerobic capacity, endurance, and fitness. This study investigated the effects of an eight-week free sprint training program on haemoglobin levels, haematological markers, muscle damage, and performance in male university football players.
METHODS:
Forty-two participants (age: 27.3 ± 3.4 years; height: 176.2 ± 6.5 cm; body mass: 71.3 ± 6.1 kg) were randomly assigned to a sprint training group (n = 21) or a control group (n = 21). The training group completed structured sprint sessions thrice weekly for eight weeks, while the control group continued their usual activity. Pre- and post-training assessments included haemoglobin concentration, neutrophil and basophil percentages, creatine kinase muscle isoenzyme (CK-MM), agility (T-test), and 30-meter sprint performance. Blood parameters were analysed using a Haematology Analyzer HS600 and BS-240 Pro. Data were tested for normality (Shapiro–Wilk), and paired t-tests assessed within-group differences.
RESULTS:
The sprint training group showed a significant increase in haemoglobin (P < 0.0001; mean difference = 1.294 g/dL) with no change in controls. CK-MM significantly decreased (P = 0.045), suggesting reduced muscle damage. Agility (P = 0.003) and 30-m sprint performance (P = 0.006) improved significantly following the intervention. No significant changes were observed in the control group.
CONCLUSION:
These findings suggest that free sprint training can enhance haematological parameters, reduce muscle damage, and improve speed and agility in collegiate football players. Incorporating sprint training may offer an effective, time-efficient strategy for performance enhancement and physiological conditioning in team sport athletes.